Part:BBa_K2768007:Design

sapd

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 477
  Illegal NgoMIV site found at 864
  Illegal AgeI site found at 667
  Illegal AgeI site found at 826
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We did the codon optimization, referring to E.coli K12

Source

Sapd sequence: https://www.ncbi.nlm.nih.gov/protein/WP_044040031.1?report=genpept Pseudomonas putida S16 (strain: S16) genomic sequence: https://www.ncbi.nlm.nih.gov/nuccore/NC_015733.1?report=genbank